Genetic Deletion of Laminin Isoforms β2 and γ3 Induces a Reduction in Kir4.1 and Aquaporin-4 Expression and Function in the Retina

Genetic Deletion of Laminin Isoforms β2 and γ3 Induces a Reduction in Kir4.1 and Aquaporin-4 Expression and Function in the Retina.

Hirrlinger PG, Pannicke T, Winkler U, Claudepierre T, Varshney S, et al. (2011)

PLoS ONE 6(1): e16106. doi:10.1371/journal.pone.0016106

Whole Transcriptome Sequencing Reveals Gene Expression and Splicing Differences in Brain Regions Affected by Alzheimer’s Disease

Whole Transcriptome Sequencing Reveals Gene Expression and Splicing Differences in Brain Regions Affected by Alzheimer’s Disease.

Twine NA, Janitz K, Wilkins MR, Janitz M (2011)

PLoS ONE 6(1): e16266. doi:10.1371/journal.pone.0016266

Loss of Caveolin-1 Accelerates Neurodegeneration and Aging

Loss of Caveolin-1 Accelerates Neurodegeneration and Aging

Head BP, Peart JN, Panneerselvam M, Yokoyama T, Pearn ML, et al. (2010)

PLoS ONE 5(12): e15697. doi:10.1371/journal.pone.0015697

IQGAP1 Is Involved in Post-Ischemic Neovascularization by Regulating Angiogenesis and Macrophage Infiltration

IQGAP1 Is Involved in Post-Ischemic Neovascularization by Regulating Angiogenesis and Macrophage Infiltration

Urao N, Razvi M, Oshikawa J, McKinney RD, Chavda R, et al. (2010)

PLoS ONE 5(10): e13440. doi:10.1371/journal.pone.0013440

Detection of Hypoxic Cells by Monoclonal Antibody Recognizing 2-Nitroimidazole Adducts

Detection of Hypoxic Cells by Monoclonal Antibody Recognizing 2-Nitroimidazole Adducts

Edith M. Lord, Lee Harwell, and Cameron J. Koch

CANCER RESEARCH 53, 5721-5726, December1, 1993

Role of myocardial hypoxia in the remodeling of the embryonic avian cardiac outflow tract

Role of myocardial hypoxia in the remodeling of the embryonic avian cardiac outflow tract

Yasuyuki Sugishita, Michiko Watanabe, and Steven A. Fisher

Developmental Biology 267 (2004) 294–308 doi:10.1016/j.ydbio.2003.11.017

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Two years ago, I pointed to a methodological flaw in one of the papers of Vincent Fleury, which didn’t contain data (or any consideration of) concerning the eventual contribution of hypoxia in one of the experimental settings. That was felt as a lèse majesté crime. The response was rather arrogant and this paper is a nice illustration of what I meant (and mean) about the eventual role of hypoxia. In the work of Sugishita et al. the hypoxic region is embedded in tissue otherwise normoxic. The red label (Cy3) pointed by the arrowheads indicate adducts by EF5, produced preferentially under hypoxic conditions.

So much for Fleury’s comment
This is the case for a normally developing embryo without any artificial occlusion impairing blood’s flow and oxygenation. Just imagine what kind of staining must result from impairing the blood flow!

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Now, what are the references for EF5 and the anti-EF5 monoclonals?

Dynamics of endothelial cell behavior in sprouting angiogenesis

Dynamics of endothelial cell behavior in sprouting angiogenesis

Hanna M Eilken, Ralf H Adams

Current Opinion in Cell Biology, In Press, Corrected Proof, Available online 2 September 2010, ISSN 0955-0674, DOI: 10.1016/j.ceb.2010.08.010.

Inflammation Dysregulates Notch Signaling In Endothelial Cells: Implication Of Notch2 And Notch4 To Endothelial Dysfunction

Inflammation Dysregulates Notch Signaling In Endothelial Cells: Implication Of Notch2 And Notch4 To Endothelial Dysfunction

Thibaut Quillard, a, Julie Devallière, Stéphanie Coupel and Béatrice Charreau

Biochemical Pharmacology Article in Press, Accepted Manuscript doi:10.1016/j.bcp.2010.07.010

Sustained release of VEGF through PLGA microparticles improves vasculogenesis and tissue remodeling in an acute myocardial ischemia-reperfusion model

Sustained release of VEGF through PLGA microparticles improves vasculogenesis and tissue remodeling in an acute myocardial ischemia-reperfusion model

Fabio R. Formiga, Beatriz Pelacho, Elisa Garbayo, Gloria Abizanda, Juan J. Gavira, Teresa Simon-Yarza, Manuel Mazo, Esther Tamayo, Carlos Jauquicoa, Carlos Ortiz-de-Solorzano, Felipe Prosper, Maria J. Blanco-Prieto

Journal of Controlled Release Article in Press, Accepted Manuscript doi:10.1016/j.jconrel.2010.07.097

CD40: Novel Association with Crohn’s Disease and Replication in Multiple Sclerosis Susceptibility

CD40: Novel Association with Crohn’s Disease and Replication in Multiple Sclerosis Susceptibility

Fiona Blanco-Kelly, Fuencisla Matesanz, Antonio Alcina, María Teruel, Lina M. Díaz-Gallo, María Gómez-García, Miguel A. López-Nevot, Luis Rodrigo, Antonio Nieto, Carlos Cardeña, Guillermo Alcain, Manuel Díaz-Rubio, Emilio G. de la Concha, Oscar Fernandez, Rafael Arroyo, Javier Martín, Elena Urcelay

PLoS ONE 5(7): e11520. doi:10.1371/journal.pone.0011520