Articles tagués ESC

hESCs – help them to get out of the USA

ADF is a legal alliance of Christian attorneys and like-minded organizations defending the right of people to freely live out their faith. Launched in 1994, ADF employs a unique combination of strategy, training, funding, and litigation to protect and preserve religious liberty, the sanctity of life, marriage, and the family.

That’s the presentation of ADF at the bottom of their « PR detail » concerning the recently issued preliminary injunction concerning USA federal funding of human embryonic stem cell [hESC] research.

Lire la suite »

Publicités

, , , ,

Poster un commentaire

Wnt Signaling Mediates Self-Organization and Axis Formation in Embryoid Bodies

Wnt Signaling Mediates Self-Organization and Axis Formation in Embryoid Bodies

Derk ten Berge, Wouter Koole, Christophe Fuerer, Matt Fish, Elif Eroglu and Roel Nusse

Cell Stem Cell, Volume 3, Issue 5, 6 November 2008, Pages 508-518 doi:10.1016/j.stem.2008.09.013

Lire la suite »

, , , ,

Poster un commentaire

In Vitro Differentiation of Mouse Embryonic Stem Cells Into Primitive Blood Vessels

In Vitro Differentiation of Mouse Embryonic Stem Cells Into Primitive Blood Vessels

Svetlana N. Rylova, Paramjeet K. Randhawa, and Victoria L. Bautch

Methods in Enzymology, Volume 443 DOI: 10.1016/S0076-6879(08)02006-5

Mouse embryonic stem (ES) cells, derived from the inner cell mass of blastocyst stage embryos, undergo programmed differentiation in vitro to form a primitive vasculature. This programmed differentiation proceeds through similar processes of vasculogenesis and angiogenesis found during early vascular development in vivo. Partially differentiated ES cell clumps or embryoid bodies (EBs) first form blood islands that are subsequently transformed into a network of primitive blood vessels that contain lumens. Therefore, vascular differentiation of ES cells is an ideal model to study and manipulate early vascular development. Here we provide protocols for the routine maintenance of mouse ES cells and in vitro differentiation. We also include protocols for establishing transgenic ES cell lines and visualization of blood vessels by use of endothelial specific molecular markers.

Lire la suite »

, , ,

Poster un commentaire