Articles tagués ESC
ADF is a legal alliance of Christian attorneys and like-minded organizations defending the right of people to freely live out their faith. Launched in 1994, ADF employs a unique combination of strategy, training, funding, and litigation to protect and preserve religious liberty, the sanctity of life, marriage, and the family.
That’s the presentation of ADF at the bottom of their « PR detail » concerning the recently issued preliminary injunction concerning USA federal funding of human embryonic stem cell [hESC] research.
Derk ten Berge, Wouter Koole, Christophe Fuerer, Matt Fish, Elif Eroglu and Roel Nusse
Cell Stem Cell, Volume 3, Issue 5, 6 November 2008, Pages 508-518 doi:10.1016/j.stem.2008.09.013
In Vitro Differentiation of Mouse Embryonic Stem Cells Into Primitive Blood Vessels
Svetlana N. Rylova, Paramjeet K. Randhawa, and Victoria L. Bautch
Methods in Enzymology, Volume 443 DOI: 10.1016/S0076-6879(08)02006-5
Mouse embryonic stem (ES) cells, derived from the inner cell mass of blastocyst stage embryos, undergo programmed differentiation in vitro to form a primitive vasculature. This programmed differentiation proceeds through similar processes of vasculogenesis and angiogenesis found during early vascular development in vivo. Partially differentiated ES cell clumps or embryoid bodies (EBs) first form blood islands that are subsequently transformed into a network of primitive blood vessels that contain lumens. Therefore, vascular differentiation of ES cells is an ideal model to study and manipulate early vascular development. Here we provide protocols for the routine maintenance of mouse ES cells and in vitro differentiation. We also include protocols for establishing transgenic ES cell lines and visualization of blood vessels by use of endothelial specific molecular markers.