Archives de la catégorie bacteria

The Repertoire of ICE in Prokaryotes Underscores the Unity, Diversity, and Ubiquity of Conjugation

The Repertoire of ICE in Prokaryotes Underscores the Unity, Diversity, and Ubiquity of Conjugation.

Guglielmini J, Quintais L, Garcillán-Barcia MP, de la Cruz F, Rocha EPC (2011)

PLoS Genet 7(8): e1002222. doi:10.1371/journal.pgen.100222210.1371/journal.pgen.1002222

94E66862-A26C-4490-8BC2-B242DA0513A7.jpg
Scheme of some essential interactions in the process of ICEs movement.

The integration/excision reaction (1) occurs by recombination across two recombination sites (yellow squares) located at the termini of the inserted element. As a result, a circular (most commonly non-replicating) DNA molecule is produced. Conjugation (2) is carried out by mobility systems. The relaxase (red circle) cleaves a specific site within oriT, and this step starts conjugation. The DNA strand that contains the relaxase protein covalently bound to its 5′-end is displaced by an ongoing conjugative DNA replication process (dotted lines). The relaxase interacts with the T4CP (green oval) and then with other components of the T4SS (blue rectangle). As a result, the relaxase-DNA complex is transported to the recipient cell [13]. Since ICEs are supposed not to replicate autonomously, the process terminates necessarily by integration of the transferred DNA circle in the recipient chromosome.

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Evolution of restraint in a structured rock–paper–scissors community

Evolution of restraint in a structured rock–paper–scissors community

Joshua R. Nahum, Brittany N. Harding, and Benjamin Kerr

PNAS , Published online before print June 20, 2011, doi: 10.1073/pnas.1100296108

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Robust multicellular computing using genetically encoded NOR gates and chemical ‘wires’

Robust multicellular computing using genetically encoded NOR gates and chemical ‘wires’

Alvin Tamsir, Jeffrey J. Tabor & Christopher A. Voigt

Nature (2010) doi:10.1038/nature09565

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Emergent cooperation in microbial metabolism

Emergent cooperation in microbial metabolism

Edwin H Wintermute & Pamela A Silver

Molecular Systems Biology 6 Article number: 407 doi:10.1038/msb.2010.66

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Box 1. Cocultures identify synergistic growth
We selected 46 conditionally lethal metabolic mutants from the Keio collection and labeled them with plasmids expressing Venus or mCherry fluorescent protein. Strains were co-cultured in all pairwise combinations in minimal medium in which no strain could grow independently.

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A Forward-Genetic Screen and Dynamic Analysis of Lambda Phage Host-Dependencies Reveals an Extensive Interaction Network and a New Anti-Viral Strategy

A Forward-Genetic Screen and Dynamic Analysis of Lambda Phage Host-Dependencies Reveals an Extensive Interaction Network and a New Anti-Viral Strategy

Nathaniel D. Maynard, Elsa W. Birch, Jayodita C. Sanghvi, Lu Chen, Miriam V. Gutschow, Markus W. Covert

PLoS Genet 6(7): e1001017. doi:10.1371/journal.pgen.1001017

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Activation of Hypoxia Inducible Factor 1 Is a General Phenomenon in Infections with Human Pathogens

Activation of Hypoxia Inducible Factor 1 Is a General Phenomenon in Infections with Human Pathogens

Nadine Werth, Christiane Beerlage, Christian Rosenberger, Amir S. Yazdi, Markus Edelmann, Amro Amr, Wanja Bernhardt, Christof von Eiff, Karsten Becker, Andrea Schäfer, Andreas Peschel, Volkhard A. J. Kempf

PLoS ONE 5(7): e11576. doi:10.1371/journal.pone.0011576

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Craig Venter unveils « synthetic life »

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Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome

Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome

Daniel G. Gibson, John I. Glass, Carole Lartigue, Vladimir N. Noskov, Ray-Yuan Chuang, Mikkel A. Algire, Gwynedd A. Benders, Michael G. Montague, Li Ma, Monzia M. Moodie, Chuck Merryman, Sanjay Vashee, Radha Krishnakumar, Nacyra Assad-Garcia, Cynthia Andrews-Pfannkoch, Evgeniya A. Denisova, Lei Young, Zhi-Qing Qi, Thomas H. Segall-Shapiro, Christopher H. Calvey, Prashanth P. Parmar, Clyde A. Hutchison, III, Hamilton O. Smith, J. Craig Venter

Published Online May 20, 2010 Science doi: 10.1126/science.1190719

We report the design, synthesis, and assembly of the 1.08-Mbp Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a Mycoplasma capricolum recipient cell to create new Mycoplasma mycoides cells that are controlled only by the synthetic chromosome. The only DNA in the cells is the designed synthetic DNA sequence, including « watermark » sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process. The new cells have expected phenotypic properties and are capable of continuous self-replication.

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50000 générations d’E.coli

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An Agent-Based Model of Signal Transduction in Bacterial Chemotaxis

An Agent-Based Model of Signal Transduction in Bacterial Chemotaxis

Jameson Miller, Miles Parker, Robert B. Bourret, Morgan C. Giddings

PLoS ONE 5(5): e9454. doi:10.1371/journal.pone.0009454

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