Macrophages programmed by apoptotic cells promote angiogenesis via prostaglandin E2

Macrophages programmed by apoptotic cells promote angiogenesis via prostaglandin E2

Kerstin Brecht, Andreas Weigert, Jiong Hu, Rüdiger Popp, Beate Fisslthaler, Thomas Korff, Ingrid Fleming, Gerd Geisslinger and Bernhard Brüne

Published online before print March 30, 2011, doi: 10.1096/fj.10-179473

Macrophages contribute to tissue homeostasis in the developing as well as the adult organism. They promote tissue regeneration and remodeling after injury, which requires efficient neoangiogenesis. Signaling pathways activating an angiogenic program in macrophages are still poorly defined. We report that apoptotic cells (ACs), which originate from stressed or damaged tissues, can induce angiogenic properties in primary human macrophages. The signal originating from ACs is the lipid mediator sphingosine-1-phosphate (S1P), which activates S1P1/3 on macrophages to up-regulate cyclooxygenase-2. The formation and liberation of prostaglandin E2 (PGE₂) then stimulates migration of endothelial cells. This is demonstrated by using PGE₂ receptor antagonists or a neutralizing PGE₂ antibody in vitro, thereby attenuating endothelial cell migration using a Boyden chamber assay. In vivo, neutralization of PGE₂ from proangiogenic macrophage supernatants blocked vessel formation into Matrigel plugs. In particular, apoptotic cancer cells shifted prostanoid formation in macrophages selectively toward PGE₂ by up-regulating cyclooxygenase-2 and microsomal prostaglandin E synthase-1 (mPGES1), while down-regulating the PGE₂-degrading enzyme 15-hydroxyprostaglandin dehydrogenase (15-PGDH) or prostaglandin-D synthase (PGDS). Angiogenic programming of macrophages by ACs, therefore, may control responses to tissue stress such as in tumors, where macrophages support cancer progression.