Archives de 13 septembre 2010
Emergent cooperation in microbial metabolism
Edwin H Wintermute & Pamela A Silver
Molecular Systems Biology 6 Article number: 407 doi:10.1038/msb.2010.66
Box 1. Cocultures identify synergistic growth
We selected 46 conditionally lethal metabolic mutants from the Keio collection and labeled them with plasmids expressing Venus or mCherry fluorescent protein. Strains were co-cultured in all pairwise combinations in minimal medium in which no strain could grow independently.