Fukushima A, Sumi T, Ishida W, Ojima A, Kajisako M, Koyanagi A, Koyama N, Yagita H.
Immunol Lett. 2008 Nov 20. [Epub ahead of print]
To induce EC, wild-type (WT) or IFN-γ-deficient (GKO) BALB/c mice were immunized with ragweed (RW) in alum followed by RW challenge in eye drops. Twenty-four hours after RW challenge, the conjunctivas, spleens and blood were harvested to evaluate conjunctival eosinophil infiltration, RW-specific cytokine production and serum Ig levels, respectively. Abs against Notch ligands (anti-Jagged1, anti-Jagged2, anti-Delta-like (Dll)1 and anti-Dll4) were injected intraperitoneally into EC-developing mice during the induction or effector phase. As a control, normal hamster IgG (nhIgG) was injected.