Loi P, Matsukawa K, Ptak G, Clinton M, Fulka J Jr, et al. (2008 ) Freeze-Dried Somatic Cells Direct Embryonic Development after Nuclear Transfer. PLoS ONE 3(8 ): e2978. doi:10.1371/journal.pone.0002978
Dans le genre “je vais conserver l’ADN à température ambiante pour m’en servir plus tard” je ne sais pas si on sait faire mieux ! Il y a des entreprises qui basent leur business sur la conservation des acides nucléiques à température ambiante qui peuvent se faire du soucis et des appareils à lyophiliser qui sont restés inactifs qui pourraient réclamer de la maintenance avant mise en service.
The natural capacity of simple organisms to survive in a dehydrated state has long been exploited by man, with lyophylization the method of choice for the long term storage of bacterial and yeast cells. More recently, attempts have been made to apply this procedure to the long term storage of blood cells. However, despite significant progress, practical application in a clinical setting is still some way off. Conversely, to date there are no reports of attempts to lyophilize nucleated somatic cells for possible downstream applications. Here we demonstrate that lyophilised somatic cells stored for 3 years at room temperature are able to direct embryonic development following injection into enucleated oocytes. These remarkable results demonstrate that alternative systems for the long-term storage of cell lines are now possible, and open unprecedented opportunities in the fields of biomedicine and for conservation strategies.
